Description

• General description: This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.
• Application: E. coli Extract Total has been used in the preparation of unilamellar vesicles[1] in lipid screening experiments of membrane protein green fluorescent protein (GFP) based fusion proteins[2] and in the reconstitution of proteoliposomes.[3]
• Biochem/physiol Actions: The E. coli lipid extract is a combination of 16, 17 and 18 carbon containing phospholipids namely, phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. It is used for preparing supported lipid bilayers.[4] The E. coli lipid extract serves as a standard for profiling phospholipids, aids in identification and taxonomic grouping of any bacterial sample.[5]
• Packaging: 5 mL Clear Glass Sealed Ampule (100500P-100mg)