Description

• Application: Guanidine hydrochloride solution has been used as a fixation buffer in genomic DNA extraction for genomic analysis.[1]
• Biochem/physiol Actions: Guanidine‐HCl (Gnd-HCl), an efficient chaotropic agent, is broadly used in RNA isolation, globular protein denaturation, and protein refolding studies as a denaturant. It is an appropriate sample buffer for the quick and effective elution of affinity-purified protein complexes and the subsequent protein digestion step.[2] Gnd-HCl is not preferred in proteomics experiments due to its hindrance with trypsin activity at low molar concentrations and tendency to precipitate when combined with sodium dodecyl sulfate (SDS).[2] GnHCl is commonly utilized to extract proteins in extracellular matrix (ECM)‐rich tissues including bone, cartilage, ligament, and tendon.[3]