50983

Guanidine thiocyanate solution

Manufacturer: Sigma Aldrich

CAS Number: 593-84-0

Synonym(S): Guanidine isothiocyanate, Guanidinium isothiocyanate, Guanidinium rhodanide, Guanidinium thiocyanate

Select a Size

Pack Size SKU Availability Price
50 ML 50983-50-ML In Stock ₹ 7,837.30
250 ML 50983-250-ML In Stock ₹ 29,422.35

50983 - 50 ML

₹ 7,837.30

In Stock

Quantity

1

Base Price: ₹ 7,837.30

GST (18%): ₹ 1,410.714

Total Price: ₹ 9,248.014

grade

for molecular biology

Quality Level

200

product line

BioUltra

concentration

~6 M in H2O

impurities

DNases, none detected RNases, none detected insoluble matter, passes filter test phosphatases, none detected proteases, none detected

refractive index

n20/D 1.474-1.483

pH

4.5-7.0 (25 °C)

anion traces

chloride (Cl-): ≤500 mg/kgsulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤1 mg/kgAs: ≤0.1 mg/kgBa: ≤1 mg/kgBi: ≤1 mg/kgCa: ≤5 mg/kgCd: ≤1 mg/kgCo: ≤1 mg/kgCr: ≤1 mg/kgCu: ≤1 mg/kgFe: ≤1 mg/kgK: ≤20 mg/kgLi: ≤1 mg/kgMg: ≤1 mg/kgMn: ≤1 mg/kgMo: ≤1 mg/kgNa: ≤500 mg/kgNi: ≤1 mg/kgPb: ≤1 mg/kgSr: ≤1 mg/kgZn: ≤1 mg/kg

absorption

cut-off at 280 nm

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Description

  • General description: Guanidine thiocyanate is a chaotropic agent that functions as a strong protein denaturant. It is widely used in RNA isolation protocols. Guanidine thiocyanate can irreversibly inactivate RNase and DNase and breaks cellular membranes. It dissolves proteins by disturbing their secondary structures. Guanidine thiocyanate also separates nucleoproteins from nucleic acids and aids the digestion of the proteins with proteases. It improves the activity of proteinase K thus making it the protease of choice.
  • Application: Guanidine thiocyanate solution has been used to extract: plasma RNA from human immunodeficiency virus (HIV)-infected and control patientstotal RNA from collected tissues (shoots, flowers, fruits, and seeds)in reverse transcription-polymerase chain reaction (RT-PCR) analysis, RNA from teratocarcinoma cell line (Tera-1)
  • Caution: possibly crystallized product can be dissolved again by slightly heating to 40-50 °C.

SAFETY INFORMATION

Pictograms

GHS05,GHS07

Signal Word

Danger

Hazard Statements

H302 + H312 + H332,H314,H412

Precautionary Statements

P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1C

Supplementary Hazards

EUH032

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves