Description

• General description: 18:1-2:0 DG, also called 1-oleoyl-2-acetyl-sn-glycerol (OADG), is a membrane permeable and synthetic analog of diacyl glycerol (DAG).[1]
• Application: 18:1-2:0 DG (1-oleoyl-2-acetyl-sn-glycerol) may be used: in the induction of superoxide generation in neutrophils[2]as a substrate for platelet-activating factor acetyl hydrolases[3]as a protein kinase C (PKC) activator in adrenal glands[4]
• Biochem/physiol Actions: 18:1-2:0 DG (OAG) halts the inhibition of glucose uptake by the phospholipase C (PLC) inhibitor, U73122.[1] It also inhibits mitochondrial permeability transition pore (MPTP) formation and prevents fatty acid release and necrosis in lung fibroblasts.[5] OAG is a protein kinase C (PKC) activator.[4] It also inhibits TWIK-related acid-sensitive (TASK)-like potassium channels in peripheral arterial chemoreceptors.[6] OAG stimulates 5-lipooxygenase (5-LO) in human polymorphonuclear leukocytes (PMNL).[7]
• Packaging: 5 mL PTFE Vial with Screw Cap (800100O-10mg)
• Storage and Stability: Diacylglycerols are conveniently stored in chloroform solutions in glass vials with PTFE-lined caps at -20°C. Under these conditions acyl migration is minimal. Avoid plastic when handling chloroform solutions.
• Other Notes: Delivery to cells: Dry samples of diacylglycerol in chloroform, using a stream of nitrogen. Dissolve the residue in an appropriate volume of ethanol or DMSO, then dilute to the desired aqueous medium.