Description

• General description: Avanti′s nitroxide spin product listing is a group of compounds designed to act as membrane probes. A variety of positions down the hydrophobic chain are labeled with the nitroxide functional groups to allow probing the membrane at various depths. These compounds have been synthesized from 1-palmitoyl-2-hydroxy-sn-glycerol-3-phosphocholine with the product being purified by column chromatography. Various n-doxyl phosphocholines have been recently used as biophysical tools to elucidate membrane trafficking with phosphatidylinositol transfer proteins[1] and as fluorescent quenchers in lipid bilayer structural studies.[2]
• Application: 16:0-5 Doxyl PC may be used:as a component in virus-like large unilamellar vesicles (VL LUVs) to quench 4-chloro-7-nitrobenz-2-oxa-1,3-diazole (NBD) fluorescence emission[7]in the preparation of multi-lamellar vesicles (MLVs) as a site-specific quencher to perform fluorescence quenching studies[8]in the preparation of spin-labelled multi-lamellar vesicles (MLVs)[9]
• Biochem/physiol Actions: Phosphatidylcholine (PC) functions as a surfactant within the mucus to form a hydrophobic surface to inhibit bacterial penetrance.[4] It is used to treat fat embolism. Phosphatidylcholine lowers the levels of cholesterol and triglycerides.[10]
• Packaging: 5 mL Clear Glass Sealed Ampule (810601C-1mg)
• Preparation Note: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies.[11] For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM.[2], Supplemental information