Description

• General description: 1-palmitoyl-2-stearoyl-(16-doxyl)-sn-glycero-3-phosphocholine (16:0-16 Doxyl PC) is a spin labelled phospholipid.[1]
• Application: 1-palmitoyl-2-stearoyl-(16-doxyl)-sn-glycero-3-phosphocholine (16:0-16 Doxyl PC) may be used:in bilayers or membranes to study the distribution of the lipid-attached doxyl electron paramagnetic resonance (EPR) spin label[4]as spin-labelled lipid in multilamellar vesicles (MLVs) to measure membrane fluidity[1]as a spin-labeled deep quencher in small unilamellar vesicles (SUVs) for nitroxide lipid quenching experiments[5]
• Packaging: 5 mL Clear Glass Sealed Ampule (810604C-1mg)
• Preparation Note: Product use: To prevent aggregation, prepare water-based solutions of 2 mM stock solutions of n-DOXYL PCs and store in plastic. Dilute stock solutions to 0.03- 0.1 mM solutions for EPR studies.[6] For liposome preparations in fluorescent quenching measurements, dissolve the doxyl lipid in 150 μl absolute ethanol for a concentration of 40.3 mM [2], Additional supplemental information.