Description

• General description: Fatty aldehydes are enzymatically converted to fatty alcohols and fatty acids. When assessing S1P lyase enzymatic activity, the estimation of fatty aldehyde stability during the reaction is required. The (2E)-hexadecenal(d5) (857461) is suitable as the internal standard for LC/MS/MS approaches to quantify the product of S1P degradation by S1P lyase, (2E)-hexadecenal; however, a natural (2E)-hexadecenal (857469) is needed to evaluate its stability during enzymatic reaction. The method describing the details of the use of both (2E)-hexadecenal(d5) and (2E)-hexadecenal for LC/MS/MS-based characterization of S1P lyase reaction is in preparation for publication by Berdyshev et al.Personal communication from Evgeny Berdyshev, Ph.D., Assistant Professor, University of Illinois at Chicago.
• Application: 16:1 aldehyde ((2E)-hexadecenal) has been used as an internal standard for extraction and detection of derivatized fatty aldehydes from HepG2 cell line by high performance liquid chromatography-electrospray ionization-quadrupole/time-of flight mass spectrometry (HPLC-ESI-QTOF).[1]
• Biochem/physiol Actions: 16:1 aldehyde is a predominant fatty aldehyde in phospholipid of bacteria like Selenomonas ruminantium, when grown on glucose and even-numbered volatile fatty acids,[2] and lactobacillus.[3] It is one of the main aldehydes in phosphatidylethanolamine plasmalogen of Ophiura sarsi[4] and choline and ethanolamine phospholipid component of bovine adipose tissue.[5]
• Packaging: 5 mL Amber Glass Screw Cap Vial (857459P-1mg)