Description

• General description: Fatty aldehydes are enzymatically converted to fatty alcohols and fatty acids. When assessing S1P lyase enzymatic activity, the estimation of fatty aldehyde stability during the reaction is required. The (2E)-hexadecenal(d5) (857461) is suitable as the internal standard for LC/MS/MS approaches to quantify the product of S1P degradation by S1P lyase, (2E)-hexadecenal; however, a natural (2E)-hexadecenal (857469) is needed to evaluate its stability during enzymatic reaction. The method describing the details of the use of both (2E)-hexadecenal(d5) and (2E)-hexadecenal for LC/MS/MS-based characterization of S1P lyase reaction is in preparation for publication by Berdyshev et al.Personal communication from Evgeny Berdyshev, Ph.D., Assistant Professor, University of Illinois at Chicago.
• Application: 16:1 aldehyde-d5 has been used as an internal standard in lipid extraction from lung tissue and liquid chromatography/with tandem mass spectrometry analysis (LC/MS/MS).[1] It has also been used as a standard in lipid extraction from plasma, derivatization of 2E-hexadecenal for quantification in quadrupole/time-of-flight (QTOF) mass spectrometer connected with a rapid-resolution-liquid-chromatography (RRLC).[2]
• Biochem/physiol Actions: 16:1 aldehyde is a predominant fatty aldehyde in phospholipid of bovine adipose tissue,[3] Lactobacillus[4] and few bacteria like Selenomonas ruminantium, when grown on glucose and even-numbered volatile fatty acids.[5] It is one of the main aldehydes in plasmalogen of notched brittle star, Ophiura sarsi.[6]
• Packaging: 5 mL Amber Glass Screw Cap Vial (857461P-1mg)