Description

• General description: DMT-2′O-Methyl-rC(ac) Phosphoramidite is one of the 2′O-Methyl RNA monomers that feature methoxy groups at the 2′-position. Themethoxy groups are perfectly stable in all conditions employed in theassembly of oligonucleotides by automated phosphoramidite synthesis, andin all standard alkaline deprotection conditions.Its key features include:High yield of crude oligonucleotidesCompatible with DNA synthesisCan be employed together with DNA or RNA phosphoramidites in thesame synthesis to produce mixmer oligonucleotides Recommended deprotection conditions are 8 hours at 55 °C usingconcentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °CPurification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as nospecial precautions are required to provide protection against nucleolyticdegradation Synthesis of 2′O-Methyl RNA oligonucleotides is similar to standard DNA synthesis but requires an elongated coupling time (recommended is 6minutes compared to 90 seconds for DNA monomers)2′O-Methyl RNA phosphoramidites are also available with fast deprotection chemistry
• Application: 2′O-Methyl RNA nucleoside including DMT-2′O-Methyl-rC(ac) Phosphoramidite can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of: Diagnostic probes Aptamer and ribozyme development Mixed 2′O-Methyl-RNA/DNA antisense molecules