Angiotensin I Converting Enzyme (ACE) Activity Assay Kit (Fluorometric)

Description

• General description: The Angiotensin I Converting Enzyme (ACE) Activity Assay is based on the cleavage of a synthetic fluorogenic peptide. The measured fluorescence is directly proportional to the ACE activity present. The reaction is summarized as follows: ACE ACE Fluorogenic substrate ———————> Cleaved ACE Substrate + fluorescent product (λex 320nm/λem 405nm) The ACE Positive Control in the kit can be used to screen for ACE inhibitors. This kit has a linear range of 1.5-110 mU of ACE activity. Angiotensin Converting Enzyme (ACE) is a key component in the renin-angiotensin system (RAS), which is well known for its role in the regulation of blood pressure, electrolyte balance, and vascular remodeling1,2 . ACE is located mainly in the capillaries of the lungs, but can also be found in endothelial and kidney epithelial cells3. This hormone system also regulates body fluids volume. Renin produces angiotensin I by cleaving several amino acids from angiotensinogen. ACE then hydrolyzes angiotensin I to yield active angiotensin II. Angiotensin II also stimulates the adrenal cortex to secrete aldosterone, which also causes an increase in blood pressure by stimulating sodium reabsorption by the kidney1. Since ACE exhibits critical functions regulating RAS, ACE inhibition has become a promising approach for drug targeting in the treatment of cardiovascular diseases such as hypertension, heart failure, and diabetic nephropathy.The detection range : 1.5-110 mU of ACE activity.
• Application: Detection of ACE1 activity in Tissue/Cell lysates, Serum, PlasmaEnables to screen for ACE inhibitors
• Features and Benefits: Our economical ACE Kit also saves your time and efforts:A simple, quick, sensitive, and direct procedure for measuring ACE levels in a variety of samples such as serum and plasmaEnables to screen for ACE inhibitorsSupportive calculator (Click here to download a calculator excel file):will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data! User friendly: No need to weigh or mix multiple reagentsQuick instruction bench card – to assure your experimental successMore experiments in one kit - contains sufficient reagents for 200 tests.
• Unit Definition: One unit of ACE is defined as the amount of enzyme that releases 1 nmol of fluorescent product from the substrate, in 1 minute, under the assay conditions, at 37 °C.This kit contains sufficient reagents for 200 tests.