CS0340
Dihydrofolate Reductase Assay Kit
1 kit sufficient for 50-100 tests
Manufacturer: Sigma Aldrich
Synonym(S): DHFR Assay Kit
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 KIT | CS0340-1-KIT | In Stock | ₹ 50,593.80 |
CS0340 - 1 KIT
In Stock
Quantity
1
Base Price: ₹ 50,593.80
GST (18%): ₹ 9,106.884
Total Price: ₹ 59,700.684
Quality Level
200
usage
kit sufficient for 50-100 tests
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... DHFR(1719)
Description
- Application: The kit is used for the detection of DHFR activity and screening of DHFR inhibitors. The assay is based on the ability of DHFR to catalyze the reversible NADPH-dependent reduction of dihydrofolic acid to tetrahydrofolic acid. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. Dihydrofolic acid+NADPH+H+ ↔ Tetrahydrofolic acid+NADP+
- Biochem/physiol Actions: DHFR (dihydrofolate reductase) is an enzyme that catalyzes a reaction essential for the biosynthesis of nucleotidic bases of DNA. Blocking the enzyme causes cell death as a result of DNA synthesis inhibition. DHFR is an excellent target for anti-tumor drugs.
- Features and Benefits: Quick and simple method. The kit contains all the reagents required for a colorimetric assay of DHFR activity in cell lysates, tissue homogenates, or column fractions of purified enzyme. The kit includes a purified enzyme for use as a positive control and screening of DHFR inhibitors.The kit includes methotrexate (MTX), a prokaryotic and eukaryotic DHFR specific inhibitor, which exhibits anti-tumor activities.The kit was tested on A431, NIH-3T3, and CHO cell lines, rat liver, kidney, brain, and skeletal muscle tissue extracts, and recombinant DHFR.
SAFETY INFORMATION
Pictograms
Signal Word
Danger
Hazard Statements
Precautionary Statements
P202 - P261 - P264 - P301 + P310 - P302 + P352 - P305 + P351 + P338
Hazard Classifications
Acute Tox. 3 Oral - Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Quality Level: 200
usage: kit sufficient for 50-100 tests
shipped in: dry ice
storage temp.: −20°C
Gene Information: human ... DHFR(1719)
Quality Level:
200
usage:
kit sufficient for 50-100 tests
shipped in:
dry ice
storage temp.:
−20°C
Gene Information:
human ... DHFR(1719)
Quality Level: 200
usage: kit sufficient for 200 tests (of 1 mL cell suspensions) kit sufficient for 40 tests (of 5 mL cell suspensions)
shipped in: wet ice
storage temp.: 2-8°C
Gene Information: __
Quality Level:
200
usage:
kit sufficient for 200 tests (of 1 mL cell suspensions) kit sufficient for 40 tests (of 5 mL cell suspensions)
shipped in:
wet ice
storage temp.:
2-8°C
Gene Information:
__
Quality Level: 200
usage: sufficient for 500 multiwell tests
shipped in: dry ice
storage temp.: −20°C
Gene Information: human ... GSTA4(2941) , GSTM1(2944)
Quality Level:
200
usage:
sufficient for 500 multiwell tests
shipped in:
dry ice
storage temp.:
−20°C
Gene Information:
human ... GSTA4(2941) , GSTM1(2944)
Quality Level: 300
usage: kit sufficient for 100 reactions (using a 1 ml cuvette) kit sufficient for 480 reactions (using 96 multiwell plates)
shipped in: dry ice
storage temp.: −20°C
Gene Information: human ... CS(1431)
Quality Level:
300
usage:
kit sufficient for 100 reactions (using a 1 ml cuvette) kit sufficient for 480 reactions (using 96 multiwell plates)
shipped in:
dry ice
storage temp.:
−20°C
Gene Information:
human ... CS(1431)