DUO82047
Duolink® In Situ Wash Buffer, Brightfield
Manufacturer: Sigma Aldrich
Synonym(S): in situ Proximity Ligation Assay Kit, Protein Protein Interaction Assay reagent
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 4 L | DUO82047-4-L | In Stock | ₹ 5,628.60 |
| 20 L | DUO82047-20-L | In Stock | ₹ 22,514.40 |
DUO82047 - 4 L
In Stock
Quantity
1
Base Price: ₹ 5,628.60
GST (18%): ₹ 1,013.148
Total Price: ₹ 6,641.748
material
packing (powdered buffer pouches)
Quality Level
200
product line
Duolink®
technique(s)
proximity ligation assay: suitable
suitability
suitable for brightfield
storage temp.
20-25°C
Related Products
Description
- Application: Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.Follow the Duolink® In Situ Brightfield Protocol to use this product.Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.To perform a complete Duolink® in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using HRP for brightfield detection.
- Features and Benefits: No overexpression or genetic manipulation requiredHigh specificity (fewer false positives)Single molecule sensitivity due to rolling circle amplificationRelative quantification possibleNo special equipment neededQuicker and simpler than FRETIncreased accuracy compared to co-IPPublication-ready results
- Legal Information: Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
SAFETY INFORMATION
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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material: packing (powdered buffer pouches)
Quality Level: 200
product line: Duolink®
technique(s): proximity ligation assay: suitable
suitability: suitable for brightfield
storage temp.: 20-25°C
material:
packing (powdered buffer pouches)
Quality Level:
200
product line:
Duolink®
technique(s):
proximity ligation assay: suitable
suitability:
suitable for brightfield
storage temp.:
20-25°C
material: packing (powdered buffer pouches)
Quality Level: 200
product line: Duolink®
technique(s): proximity ligation assay: suitable
suitability: suitable for fluorescence
storage temp.: 20-25°C
material:
packing (powdered buffer pouches)
Quality Level:
200
product line:
Duolink®
technique(s):
proximity ligation assay: suitable
suitability:
suitable for fluorescence
storage temp.:
20-25°C
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storage temp.: −20°C
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Quality Level:
100
product line:
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technique(s):
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suitability:
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storage temp.:
−20°C