Duolink® In Situ Wash Buffers, Fluorescence

Description

• Application: Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.Use the Duolink® In Situ Fluorescence Protocol for this product. A set of short instructionsis also available.Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
• Features and Benefits: No overexpression or genetic manipulation requiredHigh specificity (fewer false positives)Single molecule sensitivity due to rolling circle amplificationRelative quantification possibleNo special equipment neededQuicker and simpler than FRETIncreased accuracy compared to co-IPPublication-ready results
• Legal Information: Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany