R4268

RNA Sample Loading Buffer

for NA electrophoresis, with ethidium bromide (50 μg/mL)

Manufacturer: Sigma Aldrich

Select a Size

Pack Size SKU Availability Price
5 VIALS R4268-5-VIALS In Stock ₹ 10,667.10

R4268 - 5 VIALS

₹ 10,667.10

In Stock

Quantity

1

Base Price: ₹ 10,667.10

GST (18%): ₹ 1,920.078

Total Price: ₹ 12,587.178

grade

for molecular biology

Quality Level

200

form

liquid

concentration

1.25 ×

technique(s)

electrophoresis: suitable

foreign activity

RNase, none detected

storage temp.

−20°C−20°C

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Description

  • General description: RNA loading buffer is used as a tracking dye during RNA electrophoresis. The RNA loading dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.
  • Application: RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.
  • Components: Deionized formamide 62.5% (v/v), formaldehyde 1.14 M, bromphenol blue 200 μg/mL, xylene cyanole 200 μg/mL, MOPS-EDTA-sodium acetate at 1.25× working concentration.
  • Quantity: Recommended usage: Add 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65 °C for 10 minutes, and then chilled on ice immediately before loading on the gel.

SAFETY INFORMATION

Pictograms

GHS07,GHS08

Signal Word

Danger

Hazard Statements

H317 - H332 - H341 - H350 - H360D - H373

Precautionary Statements

P202 - P260 - P280 - P302 + P352 - P304 + P340 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Inhalation - Carc. 1B - Muta. 2 - Repr. 1B - Skin Sens. 1 - STOT RE 2 Oral

Target Organs

Blood

Storage Class Code

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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