T4415

Tris-Borate-EDTA buffer

Manufacturer: Sigma Aldrich

Synonym(S): TBE buffer

Select a Size

Pack Size SKU Availability Price
1 L T4415-1-L In Stock ₹ 5,282.78
4 L T4415-4-L In Stock ₹ 15,705.23
10 L T4415-10-L In Stock ₹ 31,410.45
20 L T4415-20-L In Stock ₹ 54,556.88

T4415 - 1 L

₹ 5,282.78

In Stock

Quantity

1

Base Price: ₹ 5,282.78

GST (18%): ₹ 950.90

Total Price: ₹ 6,233.68

Quality Level

200

product line

BioReagent

form

solution

impurities

DNase and RNase, none detected

suitability

suitable for electrophoresis

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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Description

  • General description: TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution is effective under slightly basic conditions, which keeps DNA deprotonated, water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).[1][2]
  • Application: Ready for use in gel electrophoresis after dilution to working concentrations.Tris-Borate-EDTA buffer has been used for pulsed-field gel electrophoresis (PFGE).[3]
  • Packaging: The 4L, 10L and 20L sizes are supplied in dispenser with a spigot.
  • Preparation Note: Prepared with 18 megohm water
  • Other Notes: TBE buffer is prone to precipitation over time. Precipitation generally will not adversely affect performance.

SAFETY INFORMATION

Pictograms

GHS08

Signal Word

Danger

Hazard Statements

H360FD

Precautionary Statements

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Repr. 1B

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Show Difference

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T4415

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Quality Level:
200

product line:
BioReagent

form:
solution

impurities:
DNase and RNase, none detected

suitability:
suitable for electrophoresis

SMILES string:
OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI:
1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key:
OSBLTNPMIGYQGY-UHFFFAOYSA-N

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InChI:
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SMILES string:
Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI:
1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

InChI key:
UAIUNKRWKOVEES-UHFFFAOYSA-N