T9285
Tris-EDTA buffer solution
100X concentrate for Northern and Southern blotting
Manufacturer: Sigma Aldrich
Synonym(S): Tris-EDTA wash buffer, TE buffer solution
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 100 ML | T9285-100-ML | In Stock | ₹ 3,366.58 |
T9285 - 100 ML
In Stock
Quantity
1
Base Price: ₹ 3,366.58
GST (18%): ₹ 605.984
Total Price: ₹ 3,972.564
grade
for molecular biology
Quality Level
200
sterility
0.2 μm filtered
form
liquid
pH
7.8-8.2 (1 × in water)
application(s)
sample preparation
foreign activity
DNase, RNase, none detected
storage temp.
room temp
SMILES string
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
Related Products
Description
- General description: Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.
- Application: TE buffer has been used for:isolate, resuspend, or store DNA and RNA for PCR or qPCR[1][2]gel electrophoresis, including Northern and Southern blotting[3]bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells[4]antigen retrieval for immunohistochemistry[3]
- Components: TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.
- Other Notes: For additional information on our range of Biochemicals, please complete this form.
SAFETY INFORMATION
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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grade: for molecular biology
Quality Level: 200
sterility: 0.2 μm filtered
form: liquid
pH: 7.8-8.2 (1 × in water)
application(s): sample preparation
foreign activity: DNase, RNase, none detected
storage temp.: room temp
SMILES string: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
grade:
for molecular biology
Quality Level:
200
sterility:
0.2 μm filtered
form:
liquid
pH:
7.8-8.2 (1 × in water)
application(s):
sample preparation
foreign activity:
DNase, RNase, none detected
storage temp.:
room temp
SMILES string:
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI:
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
grade: __
Quality Level: 200
sterility: __
form: powder
pH: __
application(s): __
foreign activity: __
storage temp.: −20°C
SMILES string: NC(Cc1nc[nH]n1)C(O)=O
InChI: 1S/C5H8N4O2/c6-3(5(10)11)1-4-7-2-8-9-4/h2-3H,1,6H2,(H,10,11)(H,7,8,9)
grade:
__
Quality Level:
200
sterility:
__
form:
powder
pH:
__
application(s):
__
foreign activity:
__
storage temp.:
−20°C
SMILES string:
NC(Cc1nc[nH]n1)C(O)=O
InChI:
1S/C5H8N4O2/c6-3(5(10)11)1-4-7-2-8-9-4/h2-3H,1,6H2,(H,10,11)(H,7,8,9)
grade: __
Quality Level: 200
sterility: __
form: powder
pH: __
application(s): __
foreign activity: __
storage temp.: −20°C
SMILES string: NC(Cc1nc[nH]n1)C(O)=O
InChI: 1S/C5H8N4O2/c6-3(5(10)11)1-4-7-2-8-9-4/h2-3H,1,6H2,(H,10,11)(H,7,8,9)
grade:
__
Quality Level:
200
sterility:
__
form:
powder
pH:
__
application(s):
__
foreign activity:
__
storage temp.:
−20°C
SMILES string:
NC(Cc1nc[nH]n1)C(O)=O
InChI:
1S/C5H8N4O2/c6-3(5(10)11)1-4-7-2-8-9-4/h2-3H,1,6H2,(H,10,11)(H,7,8,9)
grade: __
Quality Level: 200
sterility: __
form: aqueous solution
pH: __
application(s): __
foreign activity: __
storage temp.: −70°C
SMILES string: __
InChI: __
grade:
__
Quality Level:
200
sterility:
__
form:
aqueous solution
pH:
__
application(s):
__
foreign activity:
__
storage temp.:
−70°C
SMILES string:
__
InChI:
__