Description

• General description: Tris is an established basimetric standard and buffer used in biochemistry and molecular biology.[1] It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc.[2] It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.[3]
• Application: Tris base has been used:As a component of lysis buffer for cell disruption[4][5]In the preparation of TBE solution for PAGE (polyacrylamide gel electrophoresis)[6][5]In studies of double stranded complexes of peptide nucleic acids (PNA) and their complementary DNA sequences, by use of anion exchange HPLC[7]In capillary electrochromatography and UV analysis of tocopherols and tocotrienols[8]
• Packaging: Meets all specifications for T1503. For biotechnology, diagnostics or manufacturing use. Please inquire for commercial quantities.
• Analysis Note: Not pyrogen tested. Certificates of analysis are available with lot specific information.
• Other Notes: The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
• Legal Information: Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany