Anti-HIF-1α Antibody, clone 2B14 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 2B14 is a ZooMAb® rabbit recombinant monoclonal antibody that detects HIF-1 . It targets an epitope within 17 amino acids from the C-terminal half.
• Immunogen: KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of human HIF-1 .
• Application: Quality Control TestingEvaluated by Western Blotting in HeLa cell lysate treated with deferoxamine.A 1:10,000 dilution of this antibody detected HIF-1 in lysate from HeLa cells treated with deferoxamine (260 M, 24 h).Tested ApplicationsImmunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HIF-1 in human kidney tissue sections.Affinity Binding Assay: A representative lot of this antibody bound HIF-1 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HIF-1 in Jurkat cells (-/+ Hypoxia, 4 h).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Hypoxia-inducible factor 1-alpha (UniProt: Q16665; also known as HIF1-alpha, HIF-1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78 (bHLHe78), Member of PAS protein 1, PAS domain-containing protein 8) is encoded by the HIF1A (also known as BHLHE78, MOP1, PASD8) gene (Gene ID: 3091) in human. HIF1- is a subunit of the heterodimeric transcription factor HIF-1, which plays a central role in cellular responses to oxygen deficiency. HIF1- has several important domains: an oxygen-dependent degradation domain (ODD) that regulates its stability, a basic helix-loop-helix (bHLH) domain for DNA binding, and Per-ARNT-Sim (PAS) domains for dimerization with HIF1-β. HIF1- is crucial for the activation of genes that respond to hypoxic conditions. Under normal oxygen levels, HIF1- is rapidly degraded by the proteasome system. In hypoxia, it stabilizes and translocates to the nucleus, where it dimerizes with HIF1-β. The HIF-1 complex then binds to hypoxia-responsive elements (HREs) in the DNA, activating genes involved in angiogenesis, metabolism, iron transport, and cell survival. This response is vital for adapting to low oxygen environments and is crucial in processes like embryonic development and wound healing. Its overactivation can contribute to cancer progression by promoting angiogenesis, metabolic reprogramming, and survival under hypoxic tumor microenvironments. Conversely, its activation can be protective in ischemic diseases like myocardial infarction and stroke by promoting adaptive responses. HIF1- is also implicated in chronic kidney disease, where hypoxia is a key factor in disease progression. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.