Anti-KIAA0895L Antibody, clone 1E4 ZooMAb® Rabbit Monoclonal

Description

• General description: We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
• Specificity: Clone 1E4 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects KIAA0895L. It targets an epitope within 23 amino acids from the N-terminal half of the protein.
• Immunogen: KLH-conjugated linear peptide corresponding to 23 amino acids from the N-terminal half of human KIAA0895L.
• Application: Quality Control TestingEvaluated by Western Blotting in SH-SY5Y cell lysate.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected KIAA0895L protein in SH-SY5Y cell lysate.Tested ApplicationsImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected KIAA0895L in HeLa and A431cells.Affinity Binding Assay: A representative lot of this antibody bound KIAA0895L peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
• Target description: Microtubule-associated tyrosine carboxypeptidase 1 (UniProt: Q68EN5; also known as Microtubule-associated tyrosine carboxypeptidase) is encoded by the MATCAP1 (also known as KIAA0895L) gene (Gene ID: 653319) in human. KIAA0895L is implicated in the processing of tubulin, the main constituent of microtubules. It specifically removes the C-terminal tyrosine residue from tubulin, which is a key post-translational modification that influences microtubule stability, assembly and regulation of microtubule functions. The protein′s subcellular localization is primarily associated with microtubules and the cytoskeleton. This localization is consistent with its role in modulating microtubule dynamics. The expression of KIAA0895L is typically ubiquitous with potentially higher expression in certain tissue such as brain, indicating a possible specialized role in these tissues. Studies show that KIAA0895L/MATCAP has tubulin metallocarboxypeptidase activity and acts as -tubulin detyrosinase that also catalyzes ∆2-tubulin. Although, direct links between KIAA0895L and specific diseases are not extensively documented, its fundamental role in microtubule dynamics suggests that dysregulation of its expression or mutation could potentially be implicated in diseases associated with cellular division errors or neurodegenerative diseases. Conditions such as cancer and Alzheimer s disease, where microtubule function is disrupted, could be areas where KIAA0895L s role might be critical. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Nicot, S., et al., Sci Adv. 2023 Sep15;9(37).
• Physical form: Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
• Reconstitution: 300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
• Storage and Stability: Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
• Legal Information: ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
• Disclaimer: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.