4861485

Thermo Scientific™ Reagents for EpiJET™ Bisulfite Conversion Kit

Perform on-column desulfonation of converted DNA that has been bound to the membrane of a micro column. Thermo Scientific™ Desulfonation Buffer for EpiJET™ Bisulfite Conversion Kit provides additional reagent for use in bisulfite conversion of DNA for methylation analysis.

Manufacturer: Fischer Scientific

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For Use With (Equipment)

EpiJET Bisulfite Conversion Kit

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Description

  • Description The EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis
  • The kit integrates the thermal DNA denaturation and the bisulfite conversion reactions into one step
  • The unmethylated cytosines are deaminated and converted to uracils using powdered Modification Reagent that has been reconstituted with the two Modification Solutions
  • This step can be performed following long or short protocol, depending on the downstream application
  • The converted DNA is bound to a membrane for on-column desulfonation and subsequent purification steps, and then eluted
  • The resulting elution of the converted DNA is suitable for a number of techniques used in methylation status analysis, including PCR, qPCR, COBRA and sequencing.

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