Thermo Scientific™ EpiJET Bisulfite Conversion Kit

Description

• Thermo Scientific EpiJET Bisulfite Conversion Kit is designed for simple and reliable bisulfite conversion of DNA for methylation analysis
• In the bisulfite reaction all unmethylated cytosines are deaminated and converted to uracils, while methylated cytosines remain unchanged
• Bisulfite converted unmethylated cytosines are detected as thymines in the following PCR
• The EpiJET Bisulfite Conversion Kit integrates the thermal DNA denaturation and the bisulfite conversion reactions into one step
• This step can be performed following long or short protocol, depending on the downstream application of modified DNA
• The converted DNA is when bound to a membrane of a micro column for on-column desulfonation and subsequent DNA purification steps
• The converted DNA is eluted in a small volume of Elution Buffer and is suitable for a number of techniques used for the methylation status analysis, including PCR, qPCR, COBRA, sequencing
• Highlights • High conversion efficiency and specificity (≥99%) • Flexibility, provided by short DNA conversion protocol • Pure DNA, suitable for downstream applications and long-term storage Applications Converted DNA can be used in: • PCR • qPCR • Combined Bisulfite Restriction Analysis (COBRA) • Sequencing Includes • Modification Reagent • Modification Solution I • Modification Solution II • Binding Buffer for Bisulfite Conversion Kit • Wash buffer (conc.) for Bisulfite Conversion Kit • Desulfonation Buffer (conc.) • Elution Buffer • DNA Purification Micro Columns & Collection Tubes