Thermo Scientific™ Reagent QC Kit

Description

• Perform quality control testing of reagents that are used to identify gram-negative and gram-positive bacteria using Thermo Scientific™ Remel™ Reagent QC Kit
• The Reagent QC Kit provides positive and negative controls for evaluating specific reagents used in microbiological testing
• Reactions based on biochemical principles, resemble the conventional reactions that are found with microorganisms1
• Reagent QC Kit provides the following controls -Indole and Phenylalanine Deaminase ControlVoges-Proskauer and Nitrate Reduction ControlPeptidase (or Pyrrolidonase) ControlHippurate ControlOxidase ControlCatalase ControlBeta-Lactamase ControlNegative ControlReagent QC Kit helps to test the quality and performance of reagents used in identification of gram-negative and gram-positive bacteria using the following:Indole (IND) – Used to determine the ability of certain organisms, especially Enterobacteriaceae, to metabolize tryptophan leading to the formation of indole2
• This indole then reacts with Kovac’s indole reagent to form a red condensation product.Phenylalanine Deaminase (PDA) – Uses Ferric Chloride to detect phenylalanine deamination by Enterobacteriaceae
• Indolepyruvic acid is formed when bacteria deaminate tryptophan,3 which then reacts with ferric chloride to produce a dark brown to brown-orange color.Voges-Proskauer (VP) – VP Reagents A & B in the presence of creatine help determine acetoin production due to glucose fermentation by some Enterobacteriaceae4.Nitrate Reduction (NIT) – Uses reagents nitrite and sulfanilic acid to determine the ability of anaerobic bacteria to metabolize nitrate resulting in nitrite formation5
• Positive test is indicated by formation of a pink or red water-soluble azo dye.Peptidase (Pyrrolidonase) (PEP) – Organisms which contain pyrrolidonase split L-pyrrolidonylbeta-naphthylamide into L-pyrrolidonyl and beta-naphthylamine
• Beta-naphthylamine, which is produced by organisms, combines with the peptidase reagent to produce a red color.Hippurate (HIP) – Many bacteria have the enzyme hippuricase which splits hippuric acid and forms sodium benzoate and glycine6
• The ninhydrin reagent deaminates sodium benzoate and glycine to release ammonia, carbon dioxide, and hydrindantin
• The released ammonia reacts with residual ninhydrin and hydrindantin forms a purple substance.Oxidase (OXI) –Oxidase Reagent in the reduced form appears colorless and in the oxidized form appears dark purple
• Bacteria containing cytochrome oxidase (the enzyme responsible for the oxidation of cytochrome c) oxidize this reagent and help determine the presence or absence of oxidized cytochrome c7.Catalase (CAT) –The presence of the enzyme catalase is indicated by the production of oxygen gas bubbles when 3% hydrogen peroxide is aded8
• This helps distinguish between staphylococci and streptococci, as well as the identification of other bacteria.Beta-Lactamase (Iodometric) (BL) – The presence of beta-lactamase helps the iodine to bind to the broken lactam ring of pencillin which will result in a colorless reaction and the absence of beta-lactamase helps the iodine to combine with the starch to form a blue to black color9.Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.