Thermo Scientific™ Remel™ BactiDrop™ Desoxycholate

Description

• Differentiate between bile soluble Streptococcus pneumoniae and other insoluble alpha-hemolytic streptococci using Thermo Scientific™ Remel™ BactiDrop™ Desoxycholate (10%)
• In 1900, Neufeld used a bile salt sodium desoxycholate to detect autolysis, that differentiated bile-soluble S
• pneumoniae from bile-insoluble alpha-hemolytic streptococci1
• Evans employed 10% bile to lyse broth cultures of pneumococci2
• Hawn and Beebe evaluated the effectiveness of applying bile salts directly to a blood agar plate culture of pneumococci3
• Use Bactidrop Desoxychocolate to differentiate between bile soluble Streptococcus pneumoniae and other insoluble alpha-hemolytic streptococci.Ready-to-use: Liquid reagentEasy to interpret:Spot Test Positive Test - Colony disintegration within 30 minutesNegative Test - Colony remains intact within 30 minutesTube Test Positive Test - Clearing of Test suspension within 3 hours; Control suspension remains turbidNegative Test - Test and Control suspensions remain turbid within 3 hoursThe bile solubility test is based on the observation that when 10% desoxycholate is applied S
• pneumoniae cells lyse, while other streptococci and gram-positive cocci do not
• Bile salts cause a cell membrane derangment and also lower surface tension at the medium-membrane interface4,5.S
• pneumoniae possesses an autocatalytic enzyme that lyses the organism’s own cell wall during cell division
• This autolytic intracellular enzyme causes the organism to undergo rapid autolysis when cultivated on artificial medium6
• Goebel and Avery stated that the action of bile salts is independent of the autolytic enzyme7
• Dubos and Hirsch found that bile salts inactivate the naturally occurring autolytic enzyme either by the alteration or the removal of a normal autolytic inhibitor from the pneumococcal cells8.This reagent contains 10% w/v desoxycholic acid sodium, ethanol, sodium Merthiolate and demineralized water.Not all products are available for sale in all territories
• Please inquire.Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.