MP Biomedicals™ Trypsin from Beef pancreas

Description

• Applications: Trypsin can be used to dissolve blood clots in its microbial form and treat inflammation in its pancreatic form
• Trypsin is commonly used in biological research
• Product Description: Trypsin consists of a single chain polypeptide of 223 amino acid residues
• Trypsin is a member of the serine protease family
• Biochem/physiol Actions: Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues
• The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site
• Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids
• EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts
• Removing these ions increases the enzymatic activity
• Unit Definition: One unit hydrolyzes one µmole of TAME per minute at 25 °C, pH 8.2, in the presence of ionized calcium
• Typical Working Concentration: For the Removal of Adherent Cells from a Culture Surface: 2.5% w/v solution