Thermo Scientific™ RapidOut DNA Removal Kit

Description

• Thermo Scientific RapidOut DNA Removal Kit rapidly and safely removes genomic DNA from total RNA and mRNA preparations
• Complete digestion of DNA and safe removal of DNase I from the digestion reaction is ensured without RNA damaging steps, such as heating or organic extraction
• First, the RNA sample is treated with recombinant RNase-free DNase I to levels below the limit of detection by routine PCR
• DNase I is safely removed subsequently using proprietary DNase Removal Reagent (DRR)
• DRR efficiently binds DNase I and the complex is collected at the bottom of the tube by centrifugation
• The purified RNA is collected as a supernatant
• The RNA after RapidOut procedure is free from DNA contamination and free of DNase I
• It is ready to use in different applications including end-point or real-time RT-PCR, cloning, microarrays, and Northern blotting
• Highlights • Efficient —complete ds and ssDNA digestion and proprietary technology for DNase I removal • Rapid —single step sufficient for complete DNase I removal • Safe —no need for toxic organic extractions or RNA-damaging heating steps Main Applications • RNA isolation and RNA analysis, particularly RT-qPCR and RT-PCR (customers performing expression analysis of low transcription level genes
• • Customers performing ds-cDNA synthesis from total RNA preps
• Other applications • Elimination of DNA from RNA for microinjections and transfection
• • Elimination of DNA from RNA prior microarray analysis
• • Elimination of DNA from RNA prior Northern blot analysis.