Cut at CG^CG sites with Bsh1236I (BstUI) restriction enzyme, which performs best at 37°C in R buffer (Isoschizomers: AccII, BspFNI, BstFNI, BstUI, MvnI).
Manufacturer: Fischer Scientific
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10 U/μL
Bsh1236I (BstUI)
Yes
No
Traditional Cloning
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
10X Buffer R
37°C
Restriction Enzyme