Cut at A^CCGGT sites with BshTI (AgeI) restriction enzyme, which performs best at 37°C in O buffer (Isoschizomers: AgeI, AsiGI, CspAI, PinAI).
Manufacturer: Fischer Scientific
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10 U/μL
BshTI (AgeI)
Yes
No
Traditional Cloning
CpG Methylation-Sensitive, Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive
10X Buffer O
37°C
Restriction Enzyme