The Thermo Scientific™ Pierce™ concentrators are easy-to-use centrifugal devices that provide fast processing and excellent recovery of protein samples
These disposable ultrafiltration devices contain a polyethersulfone (PES) membrane for the concentration, desalting, and buffer exchange of biological samples, such as tissue culture media, antiserum, monoclonal antibody preparations, and chromatography fractions
They can also be used to remove unincorporated label following protein modification reactions
The PES membrane in these devices is available in five distinct molecular-weight cutoffs (MWCO) of 3K, 5K, 10K, 30K, and 100K, and can be used for processing sample volumes from 100 μL to 100 mL
The MWCOs are etched on the sides of the concentrators for easy identification, while a clear window with graduations on the side of each device allows for visual determination of the concentrated sample (retentate) volume
The unique design provides reliable and consistent results
Multiple device sizes are available to handle maximum sample volumes of 0.5 mL, 6 mL, 20 mL, and 100 mL
Rapid processing—unique design minimizes membrane fouling and sample concentration of 10- to 30-fold can be achieved in 5–30 minutes for 10K MWCO (device dependent—times may vary for other MWCO’s), even with particle laden solutions
High recovery—retain >90% of protein samples while removing contaminants or exchanging buffers
Convenient—clear markings, wide sample chamber and removable filtrate chamber make handling simple and easy
Instrument compatible—can be used with standard centrifuges utilizing either fixed-angle or swinging-bucket rotors.