Thermo Scientific™ Pierce™ Protein Concentrators PES, 10K MWCO, 0.5–100 mL

Description

• Quickly concentrate proteins and biological samples with Thermo Scientific Pierce Protein Concentrators PES (polyethersulfone), which are disposable ultrafiltration centrifugation devices that enable fast sample recovery and a short processing time
• These concentrator columns can be used with standard microcentrifuge equipment, and samples can be collected without the need for invert spinning
• Samples can be concentrated with or without desalting
• These concentrators have a molecular weight cutoff (MWCO) of 10K and can be used for processing volumes from 0.5 to 100 mL
• For protein and other biological samples, such as nucleic acids, with a 10K MWCO, Pierce Protein Concentrators PES offer rapid protein concentration, excellent sample recovery, and instrument compatibility
• These centrifugal devices feature a high-flux polyethersulfone (PES) membrane for concentration, desalting, and buffer exchange of biological samples
• These concentrators have a MWCO of 10K and can be used for processing sample volumes from 0.5 to 100 mL
• Features of 10K MWCO Protein Concentrators PES include: • Rapid protein concentration —sample concentration of 10- to 30-fold can be achieved in 10 or fewer minutes using the 0.5-mL concentrator and 15 or fewer minutes using the other quantity concentrators • Excellent protein recovery —typical protein recovery is >90% (when the sample mass is at least two-fold greater than the posted MWCO) • Instrument-compatibility —a standard microcentrifuge with swinging (except 88513) or fixed-angle rotors can be used; sample can be collected and recovered without invert spinning • Versatile —easily perform sample concentration, diafiltration, or buffer-exchange of biological samples For easy identification, the MWCO value is etched on the side of each device
• To assist with sample addition and recovery, a clear window with graduations is located on the side of each device, enabling visual determination of the retentate volume
• The vertical design of the membrane allows high-flux rates, low non-specific binding, and negligible protein aggregation on the membrane, giving reliable and consistent results for processing sample volumes between 0.5 and 100 mL
• Typical protein recovery is 90% when the mass of the proteins in the sample is at least two-fold greater than the MWCO of the device used
• For example, when concentrating IgG (MW ∼150 KDa) samples, use the 3K, 10K, 30K, or 50K MWCO concentrators
• Final sample concentrations of 10- to 30-fold can be achieved within 10 minutes when using the 0.5 mL concentrators and within 15 minutes when using the other concentrator sizes
• Protein Concentrators PES applications • Protein concentration of tissue culture media, antiserum, or monoclonal antibody preparations • Concentration of protein peaks following gel-permeation chromatography • Removal of unincorporated label following protein modification reactions • Concentration and desalt/buffer-exchange after elution of protein from ion-exchange • Separation of different sized molecules Pierce Protein Concentrators PES centrifugal devices contain a membrane rated for retaining molecules with a molecular weight at least two-fold greater than the MWCO rating of the PES membrane within the device
• Reduced recovery may occur when using a concentrator with molecules smaller than the recommended mass
• Protein recovery will vary depending on the specific protein in the sample and its starting concentration
• The dead-stop volume for the 0.5 mL, 2–6 mL, 5–20 mL, and 20–100 mL concentrators is approximately 15 μL, 30 μL, 50 μL, and 350 μL, respectively
• To achieve >90% recovery of protein with the 0.5 mL concentrators, the minimum protein sample concentration should be 0.05 mg/mL
• For all other concentrators, the minimum protein sample concentration should be 0.1 mg/mL
• Optimal centrifuges and speeds The 0.5-mL concentrators can be used effectively at a maximum centrifugal force of 15,000 × g, using a fixed angle centrifuge only
• For the 2-6 mL concentrators, swinging-bucket or fixed-angle centrifuge models can be used
• Swinging bucket centrifuges capable of generating 2,000–4,000 × g and a maximum centrifugal force of 4,000 × g are recommended
• With fixed-angle centrifuges, models capable of generating 5,000–10,000 × g and a maximum centrifugal force of 8,000 × g are recommended
• For the 5-20 mL volume concentrators, swinging-bucket or fixed-angle centrifuge models can be used
• Swinging-bucket centrifuges capable of generating 2,000–5,000 × g and a maximum centrifugal force of 4,000 × g are recommended
• With fixed-angle centrifuges, models capable of generating 5,000–8,000 × g and a maximum centrifugal force of 6,000 × g are recommended
• For the 20-100 mL concentrators, only swinging-bucket centrifuges capable of generating 1,000–2,000 × g are recommended
• Use a maximum centrifugal force of 2,000 × g for the 20–100 mL concentrators
• The concentrators are not recommended for use in fixed-angle rotors
• Centrifugal force, temperature and sample volume, concentration and viscosity will affect filtration rate and require optimization of time for each application.