Thermo Scientific™ Muller-Kauffmann Tetrathionate Broth Base (Dehydrated)

Description

• Isolate salmonellae and suppress the Proteus species with Thermo Scientific™ Oxoid™ Muller-Kauffmann Tetrathionate Broth Base (Dehydrated)
• The enrichment medium contains brilliant green and ox bile to suppress the commensal organisms, thus improving the isolation of salmonellae
• Muller 1 developed this medium in 1923
• It was later modified by Kauffmann 2,3 with the addition of brilliant green and ox bile to suppress commensal organisms and thus improve the isolation of salmonellae
• The brilliant green dye used in the medium has been shown to be critical and Chroma or BDH brands should be used
• It is essential that the dye is added as directed because heating the brilliant green or attempting to incorporate it in the basal medium seriously impairs its selective action
• The addition of novobiocin at 40mg per litre of broth was described by Jeffries 4 to suppress the growth of Proteus species
• The addition of novobiocin at 40mg per litre of broth was described by Jeffries 4 to suppress the growth of Proteus species
• Muller-Kauffmann Tetrathionate Broth should not be used if Salmonella typhi is suspected
• Muller-Kauffmann Tetrathionate Broth was used in a large-scale investigation between nine laboratories in eight different countries 5
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand