Thermo Scientific™ Modified Lauryl Sulphate Tryptose Broth Base (Dehydrated)

Description

• Selectively enrich Cronobacter spp
• from milk and milk samples using Thermo Scientific™ Oxoid™ Modified Lauryl Sulphate Tryptose Broth Base (Dehydrated)
• Modified Lauryl Sulphate Tryptose Broth Base was originally described by Guillaume-Gentil 1 et al
• for the selective enrichment of Cronobacter spp
• The high salt concentration of the medium restricts the growth of other members of Enterobacteriaceae and the combination of vancomycin and sodium lauryl sulphate inhibits the growth of Gram-positive organisms commonly isolated in production environments
• Cronobacter (formerly Enterobacter sakazakii ) are Gram-negative rod-shaped members of the Family Enterobacteriaceae that have been implicated in outbreaks of disease in premature infants (neonates), causing sepsis, meningitis, and necrotising enterocolitis
• Neurological damage can be permanent, and the death rate is reported to be as high as 40-80% 2
• Cronobacter spp
• have been isolated at low levels from powdered infant formulae, and the organisms’ high tolerance to desiccation provides a competitive advantage in the dry environments of milk powder factories, increasing the risk of product contamination 3
• Use Modified Lauryl Sulphate Tryptose Broth Base for Selective Enrichment of Cronobacter spp
• from milk and milk samples
• Selective: high salt concentration and the combination of vancomycin and sodium lauryl sulphate makes the medium selective for Cronobacter ISO compliant: conforms to ISO TS/22964:2006 4 The European Union has specified limits for the levels of Cronobacter spp
• in dried infant formulae, dried dietary foods for special medical purposes and food intended for infants below six months of age, in Commission Regulation no 2073/20055
• This regulation states, for products placed on the market during their shelf-life, 30 samples of 10g have to be tested, and the food producer has to demonstrate the absence of Cronobacter in each of these samples, using ISO/TS 22964:2006 4
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• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.