Thermo Scientific™ Oxoid™Brilliance™ Listeria Agar (ISO)

Description

• Isolate, enumerate and presumptively identify Listeria species and L
• monocytogenes from food and environmental samples with the Thermo Scientific™ Oxoid™ Brilliance ™ Listeria Agar (ISO) (Dehydrated culture medium and supplements) following broth enrichment
• The medium identifies Listeria species based on their utilization of X-glucoside giving rise to blue-green colonies
• Listeria monocytogenes are further differentiated by their ability to highly produce lecithinase, producing an opaque, white halo around the colony
• Isolate, enumerate and presumptively identify Listeria species and L
• monocytogene s with Thermo Scientific™ Oxoid™ Brilliance ™ Listeria Agar (ISO) Prepare Oxoid Brilliance Listeria Agar (ISO) by adding Thermo Scientific™ Brilliance Listeria Agar (ISO) Selective Supplement (SR0257) and Thermo Scientific™ Oxoid™ Brilliance Listeria Agar (ISO) Selective Supplement (SR0258) to Oxoid Brilliance Listeria (ISO) Agar
• Use as part of the Thermo Scientific™ Listeria Precis™ Detection and Enumeration method, or as part of the ISO 11290-1:2017 and ISO 11290-2:2017 reference methods for the detection and enumeration of Listeria species and L
• monocytogenes
• Demonstrated to produce clearly differentiated colonies of Listeria within 22 hours when following the protocols for the Listeria Precis Methods or ISO 11290-1 and ISO 11290-2 methods
• Oxoid Brilliance Listeria Agar uses the chromogen X-glucoside for presumptive identification of Listeria spp
• This chromogen is cleaved by ß-glucosidase which is common to all Listeria species
• Other organisms that possess this enzyme, such as enterococci, are inhibited by the selective agents within the medium; lithium chloride, polymyxin B and nalidixic acid, whilst amphotericin inhibits the growth of any yeasts and molds present in the sample
• Listeria monocytogenes and pathogenic Listeria ivanovii are then further differentiated by their ability to produce the phospholipase enzyme, lecithinase
• This enzyme hydrolyses the lecithin in the medium, producing an opaque white halo around the colony
• The formulation and performance of Oxoid Brilliance Listeria Agar (ISO) conforms with ISO 11290-1:2017 and ISO 11290-2:2017 reference methods for the detection and enumeration of Listeria species and L
• monocytogenes
• Oxoid Brilliance Listeria Agar (ISO) has been validated as part of the Thermo Scientific™ Listeria Precis™ Methods for a broad range of samples according to ISO 16140-2 following NF validation rules when compared to the ISO 11290-1:2017 and ISO 11290 -2:2017.