Thermo Scientific™ Simmons Citrate Agar (Dehydrated)

Description

• Differentiate members of the family Enterobacteriaceae based on the utilization of citrate with Thermo Scientific™ Oxoid™ Simmons Citrate Agar (Dehydrated)
• The medium is a solidified form of Koser citrate medium which suffered from the disadvantage that false appearance of growth occurred when large inocula were employed
• Simmons 1 modified Koser's formulation by adding agar and bromothymol blue 2
• It complies with the recommendations if the APHA 3
• Simmons Citrate Agar is used wither as slopes in test tubes or as a plate medium in petri dishes
• Positive growth (i.e
• citrate utilization) produces an alkaline reaction and changes the colour of the medium from green to bright blue, while in a negative test (i.e
• no citrate utilization) the colour of the medium remains unchanged
• Escherichia coli including serotypes from epidemic infantile enteritis, as well as Shigella, Yersinia and Edwardsiella species do not grow on the medium
• Serratia and the majority of the Enterobacter, Citrobacter, Klebsiella, Proteus and Providencia species, except Morganella morganii and Klebsiella rhinoscleromatis utilize citrate and produce the characteristic blue coloration 4
• Simmons Citrate Agar may be used to differentiate citrate-positive Salmonella enteritidis and members of Salmonella subgenus II, III and IV from the citrate-negative Salmonella typhi, Salmonella paratyphi A, Salmonella pullorum and Salmonella gallinarum
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.