Thermo Scientific™ D/E (Dey-Engley) Neutralizing Broth (Dehydrated)

Description

• Neutralize chemical disinfectants to distinguish bacteriostatic and bactericidal activity in environmental sampling procedures using Thermo Scientific™ Remel™ D/E (Dey-Engley) Neutralizing Broth (Dehydrated)
• This medium was formulated by Engley and Dey in 1970 for neutralizing and testing antiseptics and disinfectants 1
• It is recommended in the Compendium of Methods for the Microbiological Examination of Foods by the American Public Health Association (APHA) for environmental sampling 2
• Neutralize chemical disinfectants to distinguish bacteriostatic and bactericidal activity in environmental sampling procedures using D/E (Dey-Engley) Neutralizing Broth
• The medium can neutralize a range of chemicals including mercurial, iodine or chlorine preparations, quaternary ammonium compounds, formaldehyde and glutaraldehyde
• Easy to Identify: Bromocresol Purple is used as an indicator for the fermentation of dextrose by shifting from purple to yellow at acidic pH
• Easy to differentiate: Subculture all tubes of D/E Neutralizing Broth to appropriate media such as D/E Agar to observe growth (viable microorganisms)
• Bacteriostatic substance – Growth from negative D/E Neutralizing Broth tubes
• Bactericidal substance – No growth from negative D/E Neutralizing Broth tubes
• Recommended in the Compendium of Methods for the Microbiological Examination of Foods by the American Public Health Association (APHA) for environmental sampling 2
• This medium contains peptone and yeast extract that provide a source of nitrogen, carbon, and vitamins
• Dextrose provides energy and sodium thiosulfate is incorporated to neutralize chlorine and iodine
• Polysorbate 80 neutralizes phenols and formalin
• Sodium thioglycolate neutralizes mercurials
• Sodium bisulfite neutralizes formaldehyde and glutaraldehyde
• Lecithin neutralizes quaternary ammonium compounds.
• This medium is suitable for environmental sampling where neutralization of the chemical is important to determine its bactericidal activity
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• Please inquire
• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.