Thermo Scientific™ MRS Broth (Dehydrated)

Description

• Isolate Lactobacillus species and differentiate gram-positive cocci using Thermo Scientific™ Remel™ MRS Broth (Dehydrated)
• In 1960, De Man, Rogosa and Sharpe modified a medium formulated earlier by Rogosa et al 1 by eliminating tomato juice and created a medium which supported the growth of slower growing lactobacilli 2
• MRS broth is named for de Man, Rogosa and Sharpe and has been used to differentiate certain strains of gram-positive cocci based on their ability to produce gas in this medium
• 3-6
• Lactic acid bacteria are Gram-positive, catalase- and oxidase-negative and are fastidious in their nutritional requirements
• Growth is enhanced considerably by microaerobic conditions
• Generally, the lactic acid bacteria show delayed growth and smaller colony size than other microorganisms
• They may be overgrown in non-selective media, especially if incubation is required for 2-4 days
• MRS Agar is designed to encourage the growth of lactic acid bacteria, which includes species of the following genera: Lactobacillus, Streptococcus, Pediococcus , and Leuconostoc
• All these species can produce lactic acid in considerable amounts
• Can be used to differentiate certain strains of gram-positive cocci
• Supports the growth of slower growing lactobacilli
• Ammonium citrate and sodium acetate inhibit the growth of certain microorganisms, gram-negative bacteria, and molds
• This medium contains gelatin peptone and beef extract that supply essential nutrients and amino acids necessary for growth of lactobacilli
• Yeast extract is a source of B-complex vitamins and dextrose provides an energy source
• Polysorbate 80 supplies fatty acids required for the metabolism of lactobacilli
• Ammonium citrate and sodium acetate inhibit the growth of certain microorganisms, gram-negative bacteria, and molds
• Not all products are available for sale in all territories
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.