Thermo Scientific™ Peptone Water (Andrade) (Dehydrated)

Description

• Carry out fermentation studies with Thermo Scientific™ Oxoid™ Peptone Water (Andrade) (Dehydrated) when added with carbohydrates and other organic compounds
• Andrade's indicator is a solution of acid-Fuchsin that when added to peptone water is colorless or slightly pink at pH 7.4
• It becomes pink at acid pH levels and yellow at alkaline pH levels (working over a pH range of 5-8)
• The peptone used in this medium is free from fermentable carbohydrates and also free from nitrates which may interfere with gas production
• Peptone Water Andrade's is a nutrient base containing Andrade's indicator to which carbohydrates and other diagnostic reagents may be added for use in fermentation studies
• The biochemical identification of organisms capable of growing in this medium is made by observing the catabolism of the various carbohydrates added to separate tubes of peptone water
• To detect fermentation, which is the production of acid and gas, a small, inverted tube (Durham fermentation tube) is inserted into the peptone water to trap any gas produced by the organism
• A single bubble in the tube is sufficient to label the organism positive
• Some organisms will utilize the sugar to produce acid only, without gas formation
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.