Thermo Scientific™ Rapid Trehalose Assimilation Broth

Description

• Rapidly identify Candida (Torulopsis) glabrata using Thermo Scientific™ Remel™ Rapid Trehalose Assimilation Broth
• In 1985, Stockman and Roberts described the use of rapid assimilation trehalose broth to screen germ-tube-negative yeast isolates for the presumptive identification of C
• glabrata 1
• The test was performed in a microtiter format and interpreted after 1 hour incubation at 35° C
• The format and formulation of this screening method have been modified to accommodate the use of a tubed medium
• Candida glabrata represents approximately 20% of all yeast recovered from urine cultures 2
• Recently, C
• glabrata has emerged as an opportunistic pathogen with cases of endocarditis, meningitis and disseminated infections
• Ready to use – Convenience of a prepared media Rapid results – Results are obtained within 1-3 hours Easy to identify – Bromcresol green dye turns to yellow to yellow-green color development shows a positive result The incubation temperature is increased to 42°C to improve specificity and to facilitate rapid results This medium contains higher amounts of yeast nitrogen and carbohydrate (trehalose) compared to the Wickerham broth formulation
• Bromocresol green is incorporated as an indicator because its lower pH range is active in a more acidic environment
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• Remel™ and Oxoid™ products are now part of the Thermo Scientific brand.