Invitrogen™ S.O.C. Medium

Description

• S.O.C
• (Super Optimal broth with Catabolite repression) Medium is used in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E
• coli
• Catabolite repression is achieved by providing glucose in the medium and thus creating optimal metabolic conditions for E
• coli
• In addition, high nutrient concentration allows cells to recover after the stress induced by transformation and achieve 2–3 fold higher efficiency as compared to recovery in LB medium
• S.O.C
• Medium formulation Composition: 2% tryptone, 0.5% yeast extract, 10 mM NaCl, 2.5 mM KCl, 10 mM MgCl 2 , 10 mM MgSO 4 , and 20 mM glucose
• S.O.C
• medium has the same formulation as the rich S.O.B
• (Super Optimal Broth) medium with additionally added 10 mM MgCl 2 and 20 mM glucose (as published by Hanahan in 1983)
• Convenient, ready-to-use format S.O.C
• Medium is supplied as 10 x 10 mL bottles of ready-to-use liquid medium
• Quality testing Each lot of S.O.C
• Medium is tested to ensure conformance with the most current approved product specifications
• This currently consists of tests for pH, osmolality, sterility, and transformation efficiency using TOP10 E
• coli
• Find the media format that fits your needs We offer many ready-to-use media formulations, such as LB Broth (Cat
• No
• 10855001) and Terrific Broth (Cat
• No
• A1374301)
• Order Info Shipping Condition: Room Temperature