DAPI
for nucleic acid staining
Manufacturer: Sigma Aldrich
CAS Number: 28718-90-3
Synonym(S): 4′,6-Diamidino-2-phenylindole dihydrochloride, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 MG | D9542-1-MG | In Stock | ₹ 1,569.63 |
| 5 MG | D9542-5-MG | In Stock | ₹ 7,772.35 |
| 10 MG | D9542-10-MG | In Stock | ₹ 12,080.70 |
| 50 MG | D9542-50-MG | In Stock | ₹ 52,544.55 |
D9542 - 1 MG
In Stock
Quantity
1
Base Price: ₹ 1,569.63
GST (18%): ₹ 282.533
Total Price: ₹ 1,852.163
grade
for molecular biology
Quality Level
300
Assay
≥98% (HPLC)
form
powder
technique(s)
transfection: suitable
solubility
H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)PBS: insoluble
ε (extinction coefficient)
30 at 263 nm in H2O at 1 mM
fluorescence
λex 340 nm; λem 488 nm (nur DAPI)λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability
suitable for fluorescence
storage temp.
2-8°C
Other Options
Related Products
Description
- General description: DAPI (4′,6-Diamidino-2-phenylindole dihydrochloride) is a cell permeable, fluorescent dye that binds to DNA.
- Application: DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
- Biochem/physiol Actions: Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
- Caution: Protect from light.
- Other Notes: 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research
SAFETY INFORMATION
Pictograms
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
Target Organs
Respiratory system
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Compare Similar Items
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grade: for molecular biology
Quality Level: 300
Assay: ≥98% (HPLC)
form: powder
technique(s): transfection: suitable
solubility: H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)PBS: insoluble
ε (extinction coefficient): 30 at 263 nm in H2O at 1 mM
fluorescence: λex 340 nm; λem 488 nm (nur DAPI)λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability: suitable for fluorescence
storage temp.: 2-8°C
grade: __
Quality Level: 200
Assay: 97.5-102.0% anhydrous basis
form: powder
technique(s): __
solubility: __
ε (extinction coefficient): __
fluorescence: __
suitability: __
storage temp.: room temp
grade: __
Quality Level: 200
Assay: 97.5-102.0% anhydrous basis
form: powder
technique(s): __
solubility: __
ε (extinction coefficient): __
fluorescence: __
suitability: __
storage temp.: room temp
