MBD0015
DAPI ready made solution
For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL
Manufacturer: Sigma Aldrich
CAS Number: 28718-90-3
Synonym(S): 4′,6-Diamidino-2-phenylindole dihydrochloride, DAPI Dye, DAPI Stain, DNA Content Counterstain, 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 ML | MBD0015-1-ML | In Stock | ₹ 8,491.50 |
| 5 ML | MBD0015-5-ML | In Stock | ₹ 39,982.20 |
| 10 ML | MBD0015-10-ML | In Stock | ₹ 71,983.50 |
| 50 ML | MBD0015-50-ML | In Stock | ₹ 2,34,332.10 |
MBD0015 - 1 ML
In Stock
Quantity
1
Base Price: ₹ 8,491.50
GST (18%): ₹ 1,528.47
Total Price: ₹ 10,019.97
Quality Level
200
form
liquid
storage condition
protect from light
concentration
1 mg/mL
technique(s)
transfection: suitable
solubility
water: soluble
fluorescence
λex 340 nm; λem 488 nm (nur DAPI)λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability
suitable for nucleic acid staining
shipped in
dry ice
storage temp.
−20°C
Other Options
Related Products
Description
- General description: DAPI, 4′,6-diamidino-2-phenylindole, is a blue fluorescent nucleic acid stain that fluoresces brightly upon selectively binding to the minor groove of double stranded DNA. Its selectivity for DNA and high cell permeability allows efficient staining of nuclei with little background from the cytoplasm. The excitation and emission wavelengths of DAPI-DNA complex are 360 nm and 460 nm, respectively.
- Application: DAPI Readymade Solution is provided at 1mg/mL concentration. It is recommended to dilute DAPI readymade solution 1:1000 with a desired buffer for a 1μg/mL working concentration (typical working concentration used in most applications is 0.1μg/mL-10μg/mL). Store DAPI solution (1mg/mL) -20°C protected from light. It is recommended to aliquot DAPI readymade solution and to avoid freeze thaw cycles.DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS).
- Biochem/physiol Actions: Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
- Features and Benefits: Ready-to-use liquid, cell permeable dye.Can be used with live cells and fixed cells.Effective for fixed-cell staining and quantitation of DNA content.Optimal Counterstain when using fluorescent antibodies during fluorescence microscopy or high-content screening (HCS) applications.
- Physical form: DAPI readymade solution is provided at 1mg/mL concentration in water
SAFETY INFORMATION
Pictograms
Signal Word
Warning
Hazard Statements
Precautionary Statements
P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
Hazard Classifications
Skin Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Show Difference
Quality Level: 200
form: liquid
storage condition: protect from light
concentration: 1 mg/mL
technique(s): transfection: suitable
solubility: water: soluble
fluorescence: λex 340 nm; λem 488 nm (nur DAPI)λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability: suitable for nucleic acid staining
shipped in: dry ice
storage temp.: −20°C
Quality Level:
200
form:
liquid
storage condition:
protect from light
concentration:
1 mg/mL
technique(s):
transfection: suitable
solubility:
water: soluble
fluorescence:
λex 340 nm; λem 488 nm (nur DAPI)λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability:
suitable for nucleic acid staining
shipped in:
dry ice
storage temp.:
−20°C
Quality Level: 200
form: liquid
storage condition: protect from light
concentration: __
technique(s): DNA extraction: suitableDNA sequencing: suitablePCR: suitable
solubility: __
fluorescence: λex 340 nm; λem 488 nm (nur DAPI)λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability: suitable for nucleic acid staining
shipped in: dry ice
storage temp.: −20°C
Quality Level:
200
form:
liquid
storage condition:
protect from light
concentration:
__
technique(s):
DNA extraction: suitableDNA sequencing: suitablePCR: suitable
solubility:
__
fluorescence:
λex 340 nm; λem 488 nm (nur DAPI)λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability:
suitable for nucleic acid staining
shipped in:
dry ice
storage temp.:
−20°C
Quality Level: 200
form: __
storage condition: __
concentration: -100 ×
technique(s): FISH: suitable
solubility: __
fluorescence: __
suitability: __
shipped in: wet ice
storage temp.: 2-8°C
Quality Level:
200
form:
__
storage condition:
__
concentration:
-100 ×
technique(s):
FISH: suitable
solubility:
__
fluorescence:
__
suitability:
__
shipped in:
wet ice
storage temp.:
2-8°C
Quality Level: 200
form: liquid
storage condition: protect from light
concentration: 100 ×
technique(s): __
solubility: __
fluorescence: __
suitability: suitable for (Media supplement)
shipped in: wet ice
storage temp.: 2-8°C
Quality Level:
200
form:
liquid
storage condition:
protect from light
concentration:
100 ×
technique(s):
__
solubility:
__
fluorescence:
__
suitability:
suitable for (Media supplement)
shipped in:
wet ice
storage temp.:
2-8°C