Description

• General description: Contents25 mM solution (12.38 mg/ml), 100x concentration
• Application: Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots: Southern blot[1]Northern blot[2]Dot blotColony and plaque hybridizationGel shift assays
• Features and Benefits: CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.Save time with a fast, ultra-sensitive substrate.Easily strip and reprobe membranes.Take multiple exposures of up to two days for each experiment.Reuse the substrate by filtering and storing in sodium azide.
• Quality: Purity: Purified by HPLC.
• Preparation Note: Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stableMaleic Acid Buffer: 15 to 25 °C, stableDetection Buffer: 15 to 25 °C, stableBlocking Solution: always prepare freshlyAntibody Solution: 2 to 8 °C
• Other Notes: First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.No preincubation step required.Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.Exposure times are shorter than with CSPD.Do not use nitrocellulose membranes.Repeat exposures can be made up to two days after the addition of substrate.
• Legal Information: CDP-Star is a registered trademark of Tropix, Inc.