A11153
Invitrogen™ PichiaPink™ Secreted Vector Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A11153-Each-of-1 | In Stock | ₹ 1,18,586.16 |
A11153 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,18,586.16
GST (18%): ₹ 21,345.509
Total Price: ₹ 1,39,931.669
Format
Liquid
Promoter
AOX1
Content And Storage
PichiaPink™ Secreted Protein Vector kit contains 20 μg pPinkα-HC (the high-copy number plasmid with the S. cerevisiae Alpha mating factor presequence) with sequencing primers 5' α-factor and 3' CYC1. Store at -20°C.
Cleavage
No Cleavage Site
Shipping Condition
Room Temperature
Cloning Method
Restriction Enzyme/MCS
Inducing Agent
Methanol
Product Type
Vector Kit
Antibiotic Resistance Bacterial
Ampicillin (AmpR)
Replication Origin
pUC Origin
Protein Tag
Untagged
Vector
pPink
Related Products
Description
- The PichiaPink™ Secreted Vector Kit contains the pPINKα-HC vector for use with the PichiaPink™ Yeast Expression System
- The pPINKα-HC vector is built around the ADE2 gene for complementing the complementing the ade2 deletion in the PichiaPink™ strains
- The pPINKα-HC vector has the α-mating factor secretion signal sequence so that all proteins are secreted
- You can order the PichiaPink™ Secreted Vector Kit to refill your PichiaPink™ Yeast Expression System Kit
- The PichiaPink™ Secreted Vector Kit comes with: pPINKα-HC vector (high-copy number) (20μg) 5’α-factor sequencing primer (20μg) 3’CYC1 sequencing primer (20μg) One Shot™ TOP10™ Electrocomp™ E
- coli (Cat
- No
- C404052) Why Choose the PichiaPink™ Yeast Expression System? The PichiaPink™ Yeast Expression System is based on the yeast Pichia pastoris
- Advantages of Pichia pastoris include rapid growth, well-defined genetic background, simple media formulation, and easy handling
- For over 30 years, Pichia pastoris has been used by labs around the world for producing hundreds of different proteins from many species including human (Ref 1, 2)
- The PichiaPink™ Yeast Expression System allows convenient and cost-effective protein production from small to large scales
- For information on obtaining a commercial-use license for the PichiaPink™Yeast Expression System, please inquire at outlicensing@lifetech.com
- For Research Use Only
- Not intended for any animal or human therapeutic or diagnostic use
- Related Links Download the pPINKα-HC plasmid map (PDF)
- Learn more about the PichiaPink™ Yeast Expression System
- Learn more about our other protein expression systems
- References 1
- Cereghino JL, Cregg JM
- Heterologous protein expression in the methylotrophic yeast Pichia pastoris
- FEMS Microbiol Rev
- 2000 Jan;24(1):45-66
- [ PubMed ] 2
- Cereghino GP, Cereghino JL, Ilgen C, Cregg JM
- Production of recombinant proteins in fermenter cultures of the yeast Pichia pastoris
- Curr Opin Biotechnol
- 2002 Aug;13(4):329-32
- [ PubMed ]
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Format: Liquid
Promoter: AOX1
Content And Storage: PichiaPink™ Secreted Protein Vector kit contains 20 μg pPinkα-HC (the high-copy number plasmid with the S. cerevisiae Alpha mating factor presequence) with sequencing primers 5' α-factor and 3' CYC1. Store at -20°C.
Cleavage: No Cleavage Site
Shipping Condition: Room Temperature
Cloning Method: Restriction Enzyme/MCS
Inducing Agent: Methanol
Product Type: Vector Kit
Antibiotic Resistance Bacterial: Ampicillin (AmpR)
Replication Origin: pUC Origin
Protein Tag: Untagged
Vector: pPink
Format:
Liquid
Promoter:
AOX1
Content And Storage:
PichiaPink™ Secreted Protein Vector kit contains 20 μg pPinkα-HC (the high-copy number plasmid with the S. cerevisiae Alpha mating factor presequence) with sequencing primers 5' α-factor and 3' CYC1. Store at -20°C.
Cleavage:
No Cleavage Site
Shipping Condition:
Room Temperature
Cloning Method:
Restriction Enzyme/MCS
Inducing Agent:
Methanol
Product Type:
Vector Kit
Antibiotic Resistance Bacterial:
Ampicillin (AmpR)
Replication Origin:
pUC Origin
Protein Tag:
Untagged
Vector:
pPink
Format: __
Promoter: AOX1
Content And Storage: The PichiaPink™ Secretion Signal Set consists of 8 signal sequences which are DNA duplexes:• S. cerevisiae Alpha mating factor presequence• Asp. niger alpha Amylase• Asp. awarmori Glucoamylase signal• H. sapien serum albumin signal• K. maxianus Inulinase presequence• S.cerevisiae Killer Protein sequence• S.Cerevisiae invertase Signal sequence• G. gallus Lysozyme Signal sequenceQuantity: 40 pMolesStore at -20°C.
Cleavage: __
Shipping Condition: Room Temperature
Cloning Method: Restriction Enzyme/MCS
Inducing Agent: __
Product Type: Secretion Signal Set
Antibiotic Resistance Bacterial: __
Replication Origin: __
Protein Tag: __
Vector: pPINK-LC, pPINK-HC
Format:
__
Promoter:
AOX1
Content And Storage:
The PichiaPink™ Secretion Signal Set consists of 8 signal sequences which are DNA duplexes:• S. cerevisiae Alpha mating factor presequence• Asp. niger alpha Amylase• Asp. awarmori Glucoamylase signal• H. sapien serum albumin signal• K. maxianus Inulinase presequence• S.cerevisiae Killer Protein sequence• S.Cerevisiae invertase Signal sequence• G. gallus Lysozyme Signal sequenceQuantity: 40 pMolesStore at -20°C.
Cleavage:
__
Shipping Condition:
Room Temperature
Cloning Method:
Restriction Enzyme/MCS
Inducing Agent:
__
Product Type:
Secretion Signal Set
Antibiotic Resistance Bacterial:
__
Replication Origin:
__
Protein Tag:
__
Vector:
pPINK-LC, pPINK-HC
Format: Kit
Promoter: __
Content And Storage: Contains pouches sufficient to make 4 basic media:2 pouches PAD agar, 2 pouches YPDS, 2 pouches YPD, 2 pouches YPD agar, 1 pouch DextroseStore at room temperature
Cleavage: __
Shipping Condition: Room Temperature
Cloning Method: __
Inducing Agent: __
Product Type: __
Antibiotic Resistance Bacterial: __
Replication Origin: __
Protein Tag: __
Vector: __
Format:
Kit
Promoter:
__
Content And Storage:
Contains pouches sufficient to make 4 basic media:2 pouches PAD agar, 2 pouches YPDS, 2 pouches YPD, 2 pouches YPD agar, 1 pouch DextroseStore at room temperature
Cleavage:
__
Shipping Condition:
Room Temperature
Cloning Method:
__
Inducing Agent:
__
Product Type:
__
Antibiotic Resistance Bacterial:
__
Replication Origin:
__
Protein Tag:
__
Vector:
__
Format: __
Promoter: __
Content And Storage: Store in freezer (-5 to -30°C) and protect from light.
Cleavage: __
Shipping Condition: Room Temperature
Cloning Method: __
Inducing Agent: __
Product Type: __
Antibiotic Resistance Bacterial: __
Replication Origin: __
Protein Tag: __
Vector: __
Format:
__
Promoter:
__
Content And Storage:
Store in freezer (-5 to -30°C) and protect from light.
Cleavage:
__
Shipping Condition:
Room Temperature
Cloning Method:
__
Inducing Agent:
__
Product Type:
__
Antibiotic Resistance Bacterial:
__
Replication Origin:
__
Protein Tag:
__
Vector:
__