K512001

Invitrogen™ DES™-Inducible Kit with pCoBlast

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 K512001-Each-of-1 In Stock ₹ 2,12,188.80

K512001 - Each of 1

₹ 2,12,188.80

In Stock

Quantity

1

Base Price: ₹ 2,12,188.80

GST (18%): ₹ 38,193.984

Total Price: ₹ 2,50,382.784

Promoter

MT

Content And Storage

Each DES™-Inducible Kit contains 20 μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ,Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), frozen S2 cells, GIBCO™ Schneider'sDrosophilaMedium, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.

Protein Tag

His Tag (6x), V5 Epitope Tag

Quantity

1 kit

Product Line

DES™

Product Type

Inducible Kit

Protein Tag Position (to your gene)

C-terminal

Cloning Method

Gateway

Vector

pMT

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Description

  • Provides expression vector pMT/V5-His and a Blasticidin (pCoBlast) selection Uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to culture medium C-terminal V5 epitope tag for rapid detection with Anti-V5 Antibody C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin To facilitate cloning, set of three vectors-A, B, and C-is provided: Each vector has multiple cloning site in different reading frame relative to coding sequence of C-terminal tag Inducible Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

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K512001

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Promoter:
MT

Content And Storage:
Each DES™-Inducible Kit contains 20 μg each of a selection vector (pCoHygro or pCoBlast), pMT/V5-His A, B, C, and pMT/V5-His/lacZ,Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), frozen S2 cells, GIBCO™ Schneider'sDrosophilaMedium, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.

Protein Tag:
His Tag (6x), V5 Epitope Tag

Quantity:
1 kit

Product Line:
DES™

Product Type:
Inducible Kit

Protein Tag Position (to your gene):
C-terminal

Cloning Method:
Gateway

Vector:
pMT

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Promoter:
MT

Content And Storage:
The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider'sDrosophilaMedium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.

Protein Tag:
His Tag (6x), V5 Epitope Tag

Quantity:
1 kit

Product Line:
DES™

Product Type:
Inducible/Secreted Kit

Protein Tag Position (to your gene):
C-terminal

Cloning Method:
Gateway

Vector:
pMT