K413001
Invitrogen™ DES™-Inducible/Secreted Kit with pCoHygro
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K413001-Each-of-1 | In Stock | ₹ 2,05,344.00 |
K413001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,05,344.00
GST (18%): ₹ 36,961.92
Total Price: ₹ 2,42,305.92
Inducing Agent
Copper Sulfate
Content And Storage
The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider'sDrosophilaMedium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
Cloning Method
Restriction Enzyme/MCS
Vector
pMT
Product Type
Inducible/Secreted Kit
Protein Tag
His Tag (6x), V5 Epitope Tag
Quantity
1 kit
Product Line
DES™
Related Products
Description
- Description Offers inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency C-terminal V5 epitope tag for rapid detection with Anti-V5 Antibody C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-ch N-terminal signal sequence from insect BiP gene is provided to direct recombinant fusion protein through secretory pathway of S2 cells into culture medium Small size (3.6 kb) to improve DNA yields and increase subcloning efficiency C-terminal V5 epitope tag for rapid detection with Anti-V5 Antibody C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin To facilitate cloning, set of three vectors, A, B and C is provided; Each vector has multiple cloning site in different reading frame relative to the BiP signal sequence Inducible Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Secreted Protein Expression in Insect Cells
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Inducing Agent: Copper Sulfate
Content And Storage: The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider'sDrosophilaMedium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
Cloning Method: Restriction Enzyme/MCS
Vector: pMT
Product Type: Inducible/Secreted Kit
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 1 kit
Product Line: DES™
Inducing Agent:
Copper Sulfate
Content And Storage:
The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider'sDrosophilaMedium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
Cloning Method:
Restriction Enzyme/MCS
Vector:
pMT
Product Type:
Inducible/Secreted Kit
Protein Tag:
His Tag (6x), V5 Epitope Tag
Quantity:
1 kit
Product Line:
DES™
Inducing Agent: __
Content And Storage: Each pYES2.1 TOPO™ TA Expression Kit contains two boxes. The pYES2.1 TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pYES2.1/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers,GAL1Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Box contains all of the reagents necessary for transformation, including twenty-one 50 μl aliquots of chemically competent TOP10F´E. coli, S.O.C. medium, and supercoiled plasmid control pUC19. Store at -80°C. All components are guaranteed stable at the indicated temperature for 6 months when properly stored.
Cloning Method: TOPO-TA
Vector: pYES, TOPO-TA Cloning Vectors
Product Type: TA Yeast Expression Kit
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 20 reactions
Product Line: TOPO™, YES™
Inducing Agent:
__
Content And Storage:
Each pYES2.1 TOPO™ TA Expression Kit contains two boxes. The pYES2.1 TOPO™ TA box contains all of the reagents necessary for ligation, including 200 ng of topoisomerase I-activated pYES2.1/V5-His-TOPO™ vector, sterile water, dNTPs, 10X PCR buffer, salt solution, control template and primers,GAL1Forward and V5 C-term Reverse Primers for sequencing or PCR screening, and an expression control plasmid. Store at -20°C. The One Shot™ Box contains all of the reagents necessary for transformation, including twenty-one 50 μl aliquots of chemically competent TOP10F´E. coli, S.O.C. medium, and supercoiled plasmid control pUC19. Store at -80°C. All components are guaranteed stable at the indicated temperature for 6 months when properly stored.
Cloning Method:
TOPO-TA
Vector:
pYES, TOPO-TA Cloning Vectors
Product Type:
TA Yeast Expression Kit
Protein Tag:
His Tag (6x), V5 Epitope Tag
Quantity:
20 reactions
Product Line:
TOPO™, YES™
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