Invitrogen™ DES™-Inducible/Secreted Kit with pCoBlast
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | K513001-Each-of-1 | In Stock | ₹ 2,08,766.40 |
K513001 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,08,766.40
GST (18%): ₹ 37,577.952
Total Price: ₹ 2,46,344.352
Promoter
MT
Content And Storage
The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider'sDrosophilaMedium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
Protein Tag
His Tag (6x), V5 Epitope Tag
Quantity
1 kit
Product Line
DES™
Product Type
Inducible/Secreted Kit
Protein Tag Position (to your gene)
C-terminal
Cloning Method
Gateway
Vector
pMT
Related Products
Description
- This vector offers inducible metallothionein promoter that is induced upon addition of copper sulfate or cadmium chloride N-terminal signal sequence from insect BiP gene is provided to direct recombinant fusion protein through secretory pathway of S2 cells into culture medium pMT/BiP/V5-His vector offers small size (3.6kb) to improve DNA yields and increase subcloning efficiency C-terminal V5 epitope tag for rapid detection withAnti-V5 Antibody C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin To facilitate cloning, set of three vectors-A, B, and C-is provided: Each vector has multiple cloning site in different reading frame relative to BiP signal sequence Inducible Protein Expression in Insect Cells, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Secreted Protein Expression in Insect Cells
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Promoter: MT
Content And Storage: The DES™ Inducible/Secreted Kits contains 20 μg of each of a selection vector (pCoHygro or pCoBlast), pMT/BiP/V5-His A, B, C and pMT/BiP/V5-His/GFP, Calcium Phosphate Transfection Kit, forward and reverse primers (2 μg each), GIBCO™ Schneider'sDrosophilaMedium, frozen S2 cells, and the appropriate selection agent. Store S2 cells in liquid nitrogen. Store medium and selection agent at +4°C. Store all other reagents at -20°C. All components are guaranteed stable for 6 months when properly stored.
Protein Tag: His Tag (6x), V5 Epitope Tag
Quantity: 1 kit
Product Line: DES™
Product Type: Inducible/Secreted Kit
Protein Tag Position (to your gene): C-terminal
Cloning Method: Gateway
Vector: pMT
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