Invitrogen™ X-Gal

Description

• X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase
• This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays
• Bacteria containing active β-galactosidase produce blue colonies when grown on plates containing X-Gal
• X-Gal is supplied as white crystals and is soluble in dimethylformamide
• Primary applications Blue-white screening—detect functional β-galactosidase (reporter gene expression) Histochemical detection of β-galactosidase activity in fixed tissues X-gal overlay assay Find the media format that fits your needs We offer many ready-to-use media formulations, such as LB Broth (10855001), Terrific Broth (A1374301) or S.O.C recovery medium (15544034)
• We also offer IPTG (15529019) for blue-white screening
• Explore other media formats for bacterial growth
• Order Info Approved for shipment at Room Temperature or on Wet Ice.