4530754

Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit

Efficiently synthesize biotin-labeled DNA probes with the Thermo Scientific™ Biotin DecaLabel DNA Labeling Kit.

Manufacturer: Fischer Scientific

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Labeling Target

Genomic DNA, PCR Products, DNA (General)

No. of Reactions

10

Quantity

10 Reactions

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Description

  • Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein
  • The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C
  • Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity
  • The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template
  • You can uniformly label any length DNA fragments
  • Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems
  • Highlights Non-radioactive labeling of DNA Efficient priming of labeling reactions with random decamers High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction

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