Thermo Scientific™ DecaLabel DNA Labeling Kit

Description

• Perform fast synthesis of radiolabeled DNA probes of high specific activity with the complete system provided by Thermo Scientific™ Fermentas DecaLabel™ DNA Labeling Kit
• The kit is based on the improved random-primed method developed by Feinberg and Vogelstein (1, 2)
• The primary improvement over traditional random-primer kits involves the use of random decamers instead of hexamers to ensure more efficient annealing with DNA at 37°C
• In addition, Klenow Fragment, exo- has been genetically engineered to remove exonuclease activity
• The improved enzyme does not degrade the labeled probe during reaction and allows high labeling yields even from low template amounts
• DNA fragments of any length can be uniformly labeled
• Two labeling mixes are provided for flexibility in using either radiolabeled dATP or dCTP
• • High specific radioactivity of probes—1 x 10 9 dpm/μg DNA • Fast—5 minutes at 37°C • High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction • Flexible—suitable for either radiolabeled dATP or dCTP Recommended for: Generation of radiolabeled DNA probes for use in a variety of hybridization experiments: Southern and Northern blots, colony/plaque hybridizations, dot/slot blots and in situ hybridizations.