Biotin DecaLabel DNA Labeling Kit is an advanced system for the efficient synthesis of biotin-labeled DNA probes, based on an improved random-primed labeling method developed by Feinberg and Vogelstein
The primary improvement over traditional random-primed method involves the use of random decamers instead of hexamers, ensuring more efficient annealing with DNA at 37°C
Klenow Fragment, exo-, included in the kit, is genetically engineered enzyme with no detectable exonuclease activity
The enzyme does not degrade the labeled probe during reaction, which results in a high labeling yield even with low amounts of template
You can uniformly label any length DNA fragments
Biotin-labeled DNA is detected with the Biotin Chromogenic Detection Kit or conventional biotin-avidin or biotin-streptavidin detection systems
Highlights Non-radioactive labeling of DNA Efficient priming of labeling reactions with random decamers High yields with Klenow Fragment, exo-: no degradation of a labeled probe during reaction