12-183-016
Invitrogen™ PureLink™ RNA Micro Scale Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 12-183-016-Each-of-1 | In Stock | ₹ 38,330.88 |
12-183-016 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 38,330.88
GST (18%): ₹ 6,899.558
Total Price: ₹ 45,230.438
Content And Storage
Box 1 (PureLink™ RNA Micro Kit):• Lysis Buffer, 125 mL• Wash Buffer I, 50 mL• Wash Buffer II, 15 mL• RNase-free Water, 15.5 mL• PureLink™ Micro Kit Columns with collection tubes, 50 each• Collection Tubes, 50 each• Recovery Tubes, 50 eachBox 2 (PureLink™ DNase/Carrier RNA):• Lyophilized PureLink™ DNase, 1500 Units• PureLink™ On-Column DNase 2X Buffer, 1 mL• RNase-free Water, 1 mL• Lyophilized PureLink™ Carrier RNA, 300 μgStore all components of Box 2 at 4°C. Store remaining components at room temperature.
Purification Time
15 min. (After sample homogenization)
Sample Type
Cells, Tissue, Laser Capture Microdissection (LCM), Enzymatic Reactions
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
50 Preps
Shipping Condition
Room Temperature
Yield
Up to 17 μg
Isolation Technology
Silica Spin Column
Elution Volume
12–22 μL
Final Product Type
Total RNA
For Use With (Application)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale
Micro
Starting Material Amount
Cells: ≤105Tissue: ≤5 mgEnzymatic Reactions: ≤1.2 mL
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Description
- A simple and reliable technology for everyday RNA purification PureLink™ technology combines guanidine-isothiocyanate lysis with the speed, purity, and ease-of-use of silica-membrane purification
- The safe and easy procedure can be completed in typically less than 15 minutes with >85% recovery from the first elution
- The kit can be used for clean-up of purified RNA from enzymatic reactions, desalting of RNA samples after phenol extraction or ethanol precipitation, and concentration of RNA
- Purify RNA from up to 10 mg of tissue or 10 5 cells
- Clean up and concentrate RNA amounts ranging from pg to 100 μg in volumes as small as 10 μL
- Purified RNA is ready for use in downstream applications such as RT-PCR, qRT-PCR, northern blots, cDNA library construction, and nuclease protection assays
- A simple and rapid procedure Homogenize your sample in lysis buffer, add 70% ethanol, and mix
- Deposit the resulting solution into a PureLink Micro kit column with collection tube, and centrifuge for one minute
- Nucleic acids bind to the PureLink silica membrane in the column, and after subsequent washes and on-column digestion with DNase, purified RNA can be eluted with RNase-free water
- Effective: Expect excellent RNA recovery (up to 100 μg total RNA) from small samples Convenient and safe: The extraction procedure uses no phenol/chloroform, CsCl gradients, or precipitation with either LiCl or ethanol Fast: Obtain high-quality total RNA, typically in just 15 minutes Reliable: Obtain ready-to-use RNA suitable for any downstream application
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Content And Storage: Box 1 (PureLink™ RNA Micro Kit):• Lysis Buffer, 125 mL• Wash Buffer I, 50 mL• Wash Buffer II, 15 mL• RNase-free Water, 15.5 mL• PureLink™ Micro Kit Columns with collection tubes, 50 each• Collection Tubes, 50 each• Recovery Tubes, 50 eachBox 2 (PureLink™ DNase/Carrier RNA):• Lyophilized PureLink™ DNase, 1500 Units• PureLink™ On-Column DNase 2X Buffer, 1 mL• RNase-free Water, 1 mL• Lyophilized PureLink™ Carrier RNA, 300 μgStore all components of Box 2 at 4°C. Store remaining components at room temperature.
Purification Time: 15 min. (After sample homogenization)
Sample Type: Cells, Tissue, Laser Capture Microdissection (LCM), Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Shipping Condition: Room Temperature
Yield: Up to 17 μg
Isolation Technology: Silica Spin Column
Elution Volume: 12–22 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale: Micro
Starting Material Amount: Cells: ≤105Tissue: ≤5 mgEnzymatic Reactions: ≤1.2 mL
Content And Storage:
Box 1 (PureLink™ RNA Micro Kit):• Lysis Buffer, 125 mL• Wash Buffer I, 50 mL• Wash Buffer II, 15 mL• RNase-free Water, 15.5 mL• PureLink™ Micro Kit Columns with collection tubes, 50 each• Collection Tubes, 50 each• Recovery Tubes, 50 eachBox 2 (PureLink™ DNase/Carrier RNA):• Lyophilized PureLink™ DNase, 1500 Units• PureLink™ On-Column DNase 2X Buffer, 1 mL• RNase-free Water, 1 mL• Lyophilized PureLink™ Carrier RNA, 300 μgStore all components of Box 2 at 4°C. Store remaining components at room temperature.
Purification Time:
15 min. (After sample homogenization)
Sample Type:
Cells, Tissue, Laser Capture Microdissection (LCM), Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Shipping Condition:
Room Temperature
Yield:
Up to 17 μg
Isolation Technology:
Silica Spin Column
Elution Volume:
12–22 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale:
Micro
Starting Material Amount:
Cells: ≤105Tissue: ≤5 mgEnzymatic Reactions: ≤1.2 mL
Content And Storage: • 125 mL Lysis Buffer; store at room temperature• 50 mL Wash Buffer I; room temperature• 15 mL Wash Buffer II; room temperature• 15.5 mL RNase-free Water; room temperature• 50 PureLink Spin Cartridges with Collection Tubes; room temperature• 50 Collection Tubes; room temperature• 50 Recovery Tubes; room temperature
Purification Time: 20 min. (After sample homogenization)
Sample Type: Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 50 Preps
Shipping Condition: Room Temperature
Yield: Up to 350 μg
Isolation Technology: Silica Spin Column
Elution Volume: 30–300 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale: Mini
Starting Material Amount: Bacteria: ≤109cells, Blood: ≤0.2 mL, Cells: ≤5 x 107, Plant: ≤250 mg, Tissue: ≤200 mg, Yeast: ≤5 x 108cells, Enzymatic reactions: ≤1.2 mL
Content And Storage:
• 125 mL Lysis Buffer; store at room temperature• 50 mL Wash Buffer I; room temperature• 15 mL Wash Buffer II; room temperature• 15.5 mL RNase-free Water; room temperature• 50 PureLink Spin Cartridges with Collection Tubes; room temperature• 50 Collection Tubes; room temperature• 50 Recovery Tubes; room temperature
Purification Time:
20 min. (After sample homogenization)
Sample Type:
Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
50 Preps
Shipping Condition:
Room Temperature
Yield:
Up to 350 μg
Isolation Technology:
Silica Spin Column
Elution Volume:
30–300 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale:
Mini
Starting Material Amount:
Bacteria: ≤109cells, Blood: ≤0.2 mL, Cells: ≤5 x 107, Plant: ≤250 mg, Tissue: ≤200 mg, Yeast: ≤5 x 108cells, Enzymatic reactions: ≤1.2 mL
Content And Storage: • 20 mL Lysis Buffer; store at room temperature• 10 mL Wash Buffer I; room temperature• 4 mL Wash Buffer II; room temperature• 3 mL RNase-free Water; room temperature• 10 PureLink Spin Cartridges with Collection Tubes; room temperature• 10 Collection Tubes; room temperature• 10 Recovery Tubes; room temperature
Purification Time: 20 min. (After sample homogenization)
Sample Type: Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 10 Preps
Shipping Condition: Room Temperature
Yield: Up to 350 μg
Isolation Technology: Silica Spin Column
Elution Volume: 30–300 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale: Mini
Starting Material Amount: Bacteria: ≤109cells, Blood: ≤0.2 mL, Cells: ≤5 x 107, Plant: ≤250 mg, Tissue: ≤200 mg, Yeast: ≤5 x 108cells, Enzymatic reactions: ≤1.2 mL
Content And Storage:
• 20 mL Lysis Buffer; store at room temperature• 10 mL Wash Buffer I; room temperature• 4 mL Wash Buffer II; room temperature• 3 mL RNase-free Water; room temperature• 10 PureLink Spin Cartridges with Collection Tubes; room temperature• 10 Collection Tubes; room temperature• 10 Recovery Tubes; room temperature
Purification Time:
20 min. (After sample homogenization)
Sample Type:
Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
10 Preps
Shipping Condition:
Room Temperature
Yield:
Up to 350 μg
Isolation Technology:
Silica Spin Column
Elution Volume:
30–300 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale:
Mini
Starting Material Amount:
Bacteria: ≤109cells, Blood: ≤0.2 mL, Cells: ≤5 x 107, Plant: ≤250 mg, Tissue: ≤200 mg, Yeast: ≤5 x 108cells, Enzymatic reactions: ≤1.2 mL
Content And Storage: • 500 mL Lysis Buffer; store at room temperature• 250 mL Wash Buffer I; room temperature• 75 mL Wash Buffer II; room temperature• 75 mL RNase-free Water; room temperature• 250 PureLink Spin Cartridges with Collection Tubes; room temperature• 250 Collection Tubes; room temperature• 250 Recovery Tubes; room temperature
Purification Time: 20 min. (After sample homogenization)
Sample Type: Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 250 Preps
Shipping Condition: Room Temperature
Yield: Up to 350 μg
Isolation Technology: Silica Spin Column
Elution Volume: 30–300 μL
Final Product Type: Total RNA
For Use With (Application): RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale: Mini
Starting Material Amount: Bacteria: ≤109cells, Blood: ≤0.2 mL, Cells: ≤5 x 107, Plant: ≤250 mg, Tissue: ≤200 mg, Yeast: ≤5 x 108cells, Enzymatic reactions: ≤1.2 mL
Content And Storage:
• 500 mL Lysis Buffer; store at room temperature• 250 mL Wash Buffer I; room temperature• 75 mL Wash Buffer II; room temperature• 75 mL RNase-free Water; room temperature• 250 PureLink Spin Cartridges with Collection Tubes; room temperature• 250 Collection Tubes; room temperature• 250 Recovery Tubes; room temperature
Purification Time:
20 min. (After sample homogenization)
Sample Type:
Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
250 Preps
Shipping Condition:
Room Temperature
Yield:
Up to 350 μg
Isolation Technology:
Silica Spin Column
Elution Volume:
30–300 μL
Final Product Type:
Total RNA
For Use With (Application):
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
Scale:
Mini
Starting Material Amount:
Bacteria: ≤109cells, Blood: ≤0.2 mL, Cells: ≤5 x 107, Plant: ≤250 mg, Tissue: ≤200 mg, Yeast: ≤5 x 108cells, Enzymatic reactions: ≤1.2 mL